Fusarium proliferatum soft tissue infection at the site of a puncture by a plant: recovery, isolation, and direct molecular identification

J Clin Microbiol. 2010 Jan;48(1):338-42. doi: 10.1128/JCM.01525-09. Epub 2009 Nov 18.

Abstract

After allogeneic stem cell transplantation, a 49-year-old man developed fever and inflammation at the site of a plant puncture on a finger. A hyalohyphomycete was recovered by incubating the plant spine fragment following surgery. Amplification of the internal transcribed spacer region and 5.8S rRNA, beta-tubulin, and translation elongation factor coding genes identified Fusarium proliferatum, which was confirmed later by culture.

Publication types

  • Case Reports
  • Research Support, N.I.H., Intramural

MeSH terms

  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Eukaryotic Initiation Factors / genetics
  • Fungal Proteins / genetics
  • Fusarium / classification
  • Fusarium / genetics
  • Fusarium / growth & development
  • Fusarium / isolation & purification*
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Mycoses / diagnosis*
  • Mycoses / microbiology
  • Plants
  • RNA, Ribosomal, 5.8S / genetics
  • Sequence Analysis, DNA
  • Skin / injuries*
  • Soft Tissue Infections / microbiology*
  • Tubulin / genetics
  • Wounds, Penetrating / complications*

Substances

  • DNA, Fungal
  • DNA, Ribosomal
  • DNA, Ribosomal Spacer
  • Eukaryotic Initiation Factors
  • Fungal Proteins
  • RNA, Ribosomal, 5.8S
  • Tubulin

Associated data

  • GENBANK/GQ243744
  • GENBANK/GU168776