CFSE flow cytometric quantification of lymphocytic proliferation in extracorporeal photopheresis: use for quality control

Transfus Apher Sci. 2010 Feb;42(1):11-9. doi: 10.1016/j.transci.2009.10.002.

Abstract

Quality control is essential to validate extracorporeal photopheresis (ECP) processes. There is just one protocol based on PHA-induced proliferation. Since it involves the use of radioactive thymidine, we developed another technique using CFSE labeling. We compared the two tests in a paired series including 18 procedures. The thymidine test was valid. Once proliferation was obtained (10 patients out of 13), the CFSE test was in close agreement with it. In particular, two cases of residual proliferation after ECP were simultaneously detected by both techniques. Only the CFSE test allows targeted analysis of lymphocytes, thus identifying a surviving lymphocytic sub-population.

Publication types

  • Comparative Study
  • Validation Study

MeSH terms

  • Cell Division / drug effects
  • Cell Division / radiation effects
  • Flow Cytometry / methods*
  • Fluoresceins
  • Fluorescent Dyes / analysis*
  • Graft Rejection / blood
  • Graft Rejection / therapy
  • Graft vs Host Disease / blood
  • Graft vs Host Disease / therapy
  • Humans
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / radiation effects
  • Lymphocyte Count*
  • Lymphocyte Subsets / cytology
  • Lymphocyte Subsets / drug effects
  • Lymphocyte Subsets / radiation effects
  • Photopheresis / methods*
  • Photopheresis / standards
  • Phytohemagglutinins / pharmacology
  • Quality Control*
  • Sampling Studies
  • Sezary Syndrome / blood
  • Sezary Syndrome / therapy
  • Skin Diseases / blood
  • Skin Diseases / therapy
  • Succinimides / blood*
  • Thymidine / blood
  • Tritium / blood

Substances

  • 5-(6)-carboxyfluorescein diacetate succinimidyl ester
  • Fluoresceins
  • Fluorescent Dyes
  • Phytohemagglutinins
  • Succinimides
  • Tritium
  • Thymidine