Plasmodium cynomolgi: immunization of a rhesus monkey with exoerythrocytic stages cultured in autologous hepatocytes

Exp Parasitol. 1991 Jan;72(1):91-8. doi: 10.1016/0014-4894(91)90125-g.

Abstract

To investigate the immune response to exoerythrocytic stages of malaria parasites, a rhesus monkey was immunized with autologous primary hepatocyte cultures infected with 7-day-old liver stage parasites of Plasmodium cynomolgi. A primary antibody response against EE stage antigens was obtained, and boosted after injection of homologous viable sporozoites. Antibodies directed against sporozoites and blood stages were also detected. The polyvalent immune response observed demonstrates the antigenicity of the liver stages and suggests their involvement in the general immune response against malaria.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Protozoan / biosynthesis*
  • Antigens, Protozoan / immunology
  • Blotting, Western
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Immunization*
  • Immunohistochemistry
  • Liver / parasitology*
  • Macaca mulatta
  • Microscopy, Immunoelectron
  • Plasmodium / immunology*
  • Plasmodium / ultrastructure

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan