Abstract
We used a sensitive method based on tetramers of peptide and major histocompatibility complex II (pMHCII) to determine whether CD4(+) memory T cells resemble the T helper type 1 (T(H)1) and interleukin 17 (IL-17)-producing T helper (T(H)17) subsets described in vitro. Intravenous or intranasal infection with Listeria monocytogenes induced pMHCII-specific CD4(+) naive T cells to proliferate and produce effector cells, about 10% of which resembled T(H)1 or T(H)17 cells, respectively. T(H)1 cells were also present among the memory cells that survived 3 months after infection, whereas T(H)17 cells disappeared. The short lifespan of T(H)17 cells was associated with small amounts of the antiapoptotic protein Bcl-2, the IL-15 receptor and the receptor CD27, and little homeostatic proliferation. These results suggest that T(H)1 cells induced by intravenous infection are more efficient at entering the memory pool than are T(H)17 cells induced by intranasal infection.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Animals
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / pathology
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Cell Differentiation / immunology
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Cell Line
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Cell Proliferation
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Cell Survival / immunology
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Clone Cells / immunology
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Clone Cells / pathology
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Flow Cytometry
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / immunology
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Host-Pathogen Interactions / immunology
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Immunologic Memory / immunology
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Interferon-gamma / metabolism
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Interleukin-17 / immunology*
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Interleukin-17 / metabolism
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Listeria monocytogenes / genetics
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Listeria monocytogenes / physiology
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Listeriosis / immunology*
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Listeriosis / microbiology
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Mice
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Mice, Inbred C57BL
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Mice, Inbred Strains
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Receptors, Interleukin-15 / metabolism
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T-Lymphocytes, Helper-Inducer / immunology*
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T-Lymphocytes, Helper-Inducer / metabolism
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T-Lymphocytes, Helper-Inducer / pathology
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Th1 Cells / immunology*
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Th1 Cells / metabolism
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Th1 Cells / pathology
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Time Factors
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Tumor Necrosis Factor Receptor Superfamily, Member 7 / metabolism
Substances
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Histocompatibility Antigens Class II
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Interleukin-17
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Proto-Oncogene Proteins c-bcl-2
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Receptors, Interleukin-15
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Tumor Necrosis Factor Receptor Superfamily, Member 7
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Interferon-gamma