Detection method and toxicity study of a new disinfection by-product, 2,2,4-trichloro-5-methoxycyclopenta-4-ene-1,3-dione (TCMCD), in chlorinated drinking water

Water Res. 2010 Feb;44(3):974-80. doi: 10.1016/j.watres.2009.10.006. Epub 2009 Oct 24.

Abstract

The detection method of 2,2,4-trichloro-5-methoxycyclopenta-4-ene-1,3-dione (TCMCD), a new disinfection by-product, was established and optimized. Drinking water samples from eight cities in Jiangsu Province, China, were analyzed and TCMCD was detected ranging from 42 to 171 ng/L among the water samples from the three cities. Toxicity of TCMCD was studied using the exposure of zebrafish and the human peripheral blood lymphocyte micronucleus assay. The results showed that TCMCD is fatal to zebrafish embryos and is a potential mutagen to human beings.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • China
  • Cyclopentanes / analysis*
  • Cyclopentanes / isolation & purification
  • Cyclopentanes / toxicity*
  • Disinfection*
  • Embryo, Nonmammalian / drug effects
  • Geography
  • Halogenation / drug effects*
  • Humans
  • Hydrocarbons, Chlorinated / analysis*
  • Hydrocarbons, Chlorinated / isolation & purification
  • Hydrocarbons, Chlorinated / toxicity*
  • Micronuclei, Chromosome-Defective / drug effects
  • Mutagenicity Tests
  • Survival Analysis
  • Toxicity Tests, Acute / methods*
  • Water Supply / analysis*
  • Zebrafish / embryology

Substances

  • 2,2,4-trichloro-5-methoxycyclopent-4-ene-1,3-dione
  • Cyclopentanes
  • Hydrocarbons, Chlorinated