SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells

Cell Stem Cell. 2009 Dec 4;5(6):610-23. doi: 10.1016/j.stem.2009.10.019.

Abstract

Despite the remarkable regenerative capacity of mammalian skin, an adult dermal stem cell has not yet been identified. Here, we investigated whether skin-derived precursors (SKPs) might fulfill such a role. We show that SKPs derive from Sox2(+) hair follicle dermal cells and that these two cell populations are similar with regard to their transcriptome and functional properties. Both clonal SKPs and endogenous Sox2(+) cells induce hair morphogenesis, differentiate into dermal cell types, and home to a hair follicle niche upon transplantation. Moreover, hair follicle-derived SKPs self-renew, maintain their multipotency, and serially reconstitute hair follicles. Finally, grafting experiments show that follicle-associated dermal cells move out of their niche to contribute cells for dermal maintenance and wound-healing. Thus, SKPs derive from Sox2(+) follicle-associated dermal precursors and display functional properties predicted of a dermal stem cell, contributing to dermal maintenance, wound-healing, and hair follicle morphogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult Stem Cells / cytology
  • Adult Stem Cells / metabolism*
  • Animals
  • Animals, Newborn
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Hair Follicle / cytology*
  • Mice
  • Mice, SCID
  • Mice, Transgenic
  • Morphogenesis
  • Neurons / cytology*
  • Rats
  • Rats, Sprague-Dawley
  • Regeneration
  • SOXB1 Transcription Factors / biosynthesis*
  • Stem Cell Niche / cytology*
  • Stem Cell Transplantation
  • Wound Healing

Substances

  • SOXB1 Transcription Factors
  • Sox2 protein, mouse

Associated data

  • GEO/GSE18690