Abstract
The iclR gene of Escherichia coli K-12, which encodes a regulatory protein (repressor) for the aceBAK operon, is located between that operon and metH in the 91-min region of the chromosome. The iclR gene was cloned and expressed in a coupled T7 RNA polymerase/promoter system and the gene product was identified by specific binding to a fragment containing the aceBAK operator region. The iclR gene product is a polypeptide of 274 amino acids (aa) with a calculated Mr of 29,741. Comparison of the deduced IclR aa sequence to that of Salmonella typhimurium revealed that the two IclR repressors exhibit 89% identity. A possible helix-turn-helix motif characteristic of DNA-binding proteins was found within the IclR sequence. A search in protein data banks revealed that IclR has a score of similarity of 43.7% with GylR, a transcriptional regulator of the glycerol operon of Streptomyces coelicolor.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetates / metabolism
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Amino Acid Sequence
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Base Sequence
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Cloning, Molecular
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DNA, Bacterial / metabolism
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Gene Expression Regulation, Bacterial
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Genes, Regulator
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Molecular Sequence Data
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Operator Regions, Genetic
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Operon
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Repressor Proteins / biosynthesis
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Restriction Mapping
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Salmonella typhimurium / genetics*
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Salmonella typhimurium / metabolism
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Sequence Homology, Nucleic Acid
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Transcription Factors*
Substances
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Acetates
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Bacterial Proteins
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DNA, Bacterial
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Escherichia coli Proteins
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Repressor Proteins
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Transcription Factors
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IclR protein, E coli
Associated data
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GENBANK/M34937
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GENBANK/M63439
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GENBANK/M63440
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GENBANK/M63441
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GENBANK/M63442
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GENBANK/M63443
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GENBANK/M63444
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GENBANK/M63445
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GENBANK/M63914
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GENBANK/M74185
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GENBANK/S75878