Abstract
We evaluated a single membrane device assay for simultaneously detecting both Clostridium difficile glutamate dehydrogenase (GDH) and toxin A/B antigens against a standard that combines two PCR assays and cytotoxigenic culture. Results showing dual GDH and toxin A/B antigen positives and negatives can be reported immediately as true positives and negatives, respectively. Specimens with discrepant results for GDH and toxins A/B, which comprised 13.2% of the specimens, need to be retested.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Azure Stains / metabolism*
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Bacterial Proteins / analysis
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Bacterial Toxins / analysis
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Cell Culture Techniques / methods
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Clostridioides difficile / chemistry
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Clostridioides difficile / isolation & purification*
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Enterocolitis, Pseudomembranous / diagnosis*
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Enterotoxins / analysis
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Feces / microbiology*
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Glutamate Dehydrogenase / immunology
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Humans
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Immunoenzyme Techniques / methods*
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Methylene Blue / metabolism*
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Polymerase Chain Reaction / methods
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Sensitivity and Specificity
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Xanthenes / metabolism*
Substances
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Azure Stains
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Bacterial Proteins
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Bacterial Toxins
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Clostridium difficile lethal toxin B
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Diff Quik
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Enterotoxins
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Xanthenes
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tcdA protein, Clostridium difficile
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Glutamate Dehydrogenase
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Methylene Blue