8-OHdG in cerebrospinal fluid as a marker of oxidative stress in various neurodegenerative diseases

Neurodegener Dis. 2009;6(5-6):263-9. doi: 10.1159/000237221. Epub 2009 Dec 3.

Abstract

Background: The 8-hydroxy-2 deoxyguanosine (8-OHdG) is a product of nucleoside oxidation of DNA and a reliable marker of oxidative stress markers. Increased levels of oxidative stress have been reported in the cerebrospinal fluid (CSF) of patients with various neurodegenerative disorders.

Objective: In search of a biochemical indicator of Parkinson's disease (PD), we analyzed the levels 8-OHdG in the CSF of 99 patients, using ELISA to assess the differences between various neurodegenerative disorders.

Results: Statistically significant higher CSF levels (p = 0.022) of 8-OHdG in non-demented PD patients as compared to the control group were observed. No differences between CSF 8-OHdG levels and age at the time of lumbar puncture, presence or severity of dementia, or gender were found.

Conclusions: 8-OHdG levels could be potentially useful in the neurochemically supported diagnosis of PD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Aged
  • Aged, 80 and over
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / cerebrospinal fluid
  • Female
  • Humans
  • Male
  • Mental Status Schedule
  • Middle Aged
  • Neurodegenerative Diseases / cerebrospinal fluid*
  • Neurodegenerative Diseases / classification
  • Neurodegenerative Diseases / physiopathology*
  • Oxidative Stress / physiology*
  • Retrospective Studies

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine