A crucial step in assisted reproduction technology: human embryo selection using metabolomic evaluation

Frutos Carlos Marhuenda-Egea; Encarnación Martínez-Sabater; Rubén Gonsálvez-Alvarez; Belén Lledó; Jorge Ten; Rafael Bernabeu

doi:10.1016/j.fertnstert.2009.10.013

A crucial step in assisted reproduction technology: human embryo selection using metabolomic evaluation

Fertil Steril. 2010 Jul;94(2):772-4. doi: 10.1016/j.fertnstert.2009.10.013. Epub 2009 Dec 3.

Authors

Frutos Carlos Marhuenda-Egea¹, Encarnación Martínez-Sabater, Rubén Gonsálvez-Alvarez, Belén Lledó, Jorge Ten, Rafael Bernabeu

Affiliation

¹ Department of Agrochemistry and Biochemistry, University of Alicante, Alicante, Spain. [email protected]

PMID: 19962138
DOI: 10.1016/j.fertnstert.2009.10.013

Abstract

We present a new methodology to predict embryo viability in assisted reproductive technology (ART) treatments by determining the relative amino acid concentrations in human embryo culture medium on day 3, using high-performance liquid chromatography with mass spectroscopy analysis without derivatization. The model was performed with soft independent modeling of class analogy for the samples from nonpregnancy and pregnancy cases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / metabolism
Blastocyst / metabolism
Chromatography, Liquid
Culture Media, Conditioned / metabolism*
Female
Humans
Mass Spectrometry
Metabolome*
Models, Biological*
Pregnancy
Preimplantation Diagnosis / methods*
Reproductive Techniques, Assisted*

Substances

Amino Acids
Culture Media, Conditioned