Abstract
The lymphatic vasculature is important for the regulation of tissue fluid homeostasis, immune response, and lipid absorption, and the development of in vitro models should allow for a better understanding of the mechanisms regulating lymphatic vascular growth, repair, and function. Here we report isolation and characterization of lymphatic endothelial cells from human intestine and show that intestinal lymphatic endothelial cells have a related but distinct gene expression profile from human dermal lymphatic endothelial cells. Furthermore, we identify liprin beta1, a member of the family of LAR transmembrane tyrosine phosphatase-interacting proteins, as highly expressed in intestinal lymphatic endothelial cells in vitro and lymphatic vasculature in vivo, and show that it plays an important role in the maintenance of lymphatic vessel integrity in Xenopus tadpoles.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / metabolism*
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Animals
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cells, Cultured
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Dermis / cytology
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Endothelial Cells / cytology*
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Endothelial Cells / physiology
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Gene Knockdown Techniques
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Green Fluorescent Proteins / genetics
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Humans
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Intestinal Mucosa / cytology*
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Intracellular Signaling Peptides and Proteins
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Larva / physiology
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Lymphangiogenesis / physiology
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Lymphatic Vessels / cytology*
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Lymphatic Vessels / physiology
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Models, Animal
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Organisms, Genetically Modified
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Xenopus Proteins / genetics
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Xenopus Proteins / metabolism*
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Xenopus laevis / growth & development
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Xenopus laevis / physiology*
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Intracellular Signaling Peptides and Proteins
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PPFIBP1 protein, Xenopus
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PPFIBP1 protein, human
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Xenopus Proteins
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Green Fluorescent Proteins