The same well-characterized T cell epitope SIINFEKL expressed in the context of a cytoplasmic or secreted protein in BCG induces different CD8+ T cell responses

Immunol Lett. 2010 May 4;130(1-2):36-42. doi: 10.1016/j.imlet.2009.12.004. Epub 2009 Dec 11.

Abstract

Mycobacterium bovis BCG is still the most widely used vaccine against tuberculosis and CD8(+) T cells play important roles in fighting infection. We investigated how well antigen is processed and presented to CD8(+) T cells using the same well-characterized CD8(+) T cell epitope SIINFEKL expressed in either a cytoplasmic (GFP-OVA) or secreted (85B-OVA) context from BCG. We report that secreted SIINFEKL from 85B-OVA BCG is presented better than cytoplasmic SIINFEKL expressed by GFP-OVA BCG.

MeSH terms

  • Animals
  • BCG Vaccine / immunology
  • CD8-Positive T-Lymphocytes / immunology*
  • Cytoplasm / drug effects
  • Epitopes, T-Lymphocyte / metabolism*
  • Flow Cytometry
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Ovalbumin / metabolism*
  • Peptide Fragments / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Spleen / cytology

Substances

  • BCG Vaccine
  • Epitopes, T-Lymphocyte
  • OVA-8
  • Peptide Fragments
  • Recombinant Proteins
  • Ovalbumin