Abstract
Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Bacterial Typing Techniques / methods*
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Child
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Child, Preschool
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Cluster Analysis
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DNA Fingerprinting / methods*
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Electrophoresis, Gel, Pulsed-Field
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Female
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Genotype
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Humans
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Infant
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Male
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Molecular Epidemiology / methods
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Polymerase Chain Reaction / methods*
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Polymorphism, Genetic
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Streptococcal Infections / microbiology
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Streptococcal Infections / transmission
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Streptococcus mutans / classification*
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Streptococcus mutans / genetics*
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Streptococcus mutans / isolation & purification