aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon

Jan M Bell; John D Turnidge; Patiyan Andersson

doi:10.1128/AAC.01476-09

aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon

Antimicrob Agents Chemother. 2010 Mar;54(3):1378-80. doi: 10.1128/AAC.01476-09. Epub 2009 Dec 14.

Authors

Jan M Bell¹, John D Turnidge, Patiyan Andersson

Affiliation

¹ SA Pathology, Women's and Children's Hospital, 72 King William Rd., North Adelaide, SA 5006, Australia.

Abstract

We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6')-Ib-cr that is capable of distinguishing between the two genetic aac(6')-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.

Publication types

Evaluation Study

MeSH terms

Acetyltransferases / genetics*
Enterobacteriaceae / classification
Enterobacteriaceae / drug effects
Enterobacteriaceae / enzymology*
Genetic Variation*
Genotype
Humans
Microbial Sensitivity Tests / methods
Oligonucleotide Probes / genetics*
Polymerase Chain Reaction / economics
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length
Transition Temperature*

Substances

Oligonucleotide Probes
Acetyltransferases
aminoglycoside acetyltransferase