Direct MALDI-TOF mass spectrometric peptide profiling of neuroendocrine tissue of Drosophila

Christian Wegener; Susanne Neupert; Reinhard Predel

doi:10.1007/978-1-60761-535-4_9

Direct MALDI-TOF mass spectrometric peptide profiling of neuroendocrine tissue of Drosophila

Methods Mol Biol. 2010:615:117-27. doi: 10.1007/978-1-60761-535-4_9.

Authors

Christian Wegener¹, Susanne Neupert, Reinhard Predel

Affiliation

¹ Emmy Noether Neuropeptide Group, Animal Physiology, Philipps-University, Marburg, Germany.

PMID: 20013204
DOI: 10.1007/978-1-60761-535-4_9

Abstract

Direct MALDI-TOF mass spectrometric peptide profiling is increasingly used to analyze the peptide complement in the nervous system of a variety of invertebrate animals, from leech to Aplysia and many arthropod species, especially insects and crustaceans. Proper sample preparation is often the most crucial step to obtain the necessary data. Here, we describe protocols for the use of MALDI-TOF mass spectrometry to directly analyze the peptidome of neuroendocrine tissues of insects, particularly Drosophila melanogaster, by MALDI-TOF MS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Drosophila Proteins / analysis*
Drosophila Proteins / genetics
Drosophila melanogaster / anatomy & histology
Drosophila melanogaster / chemistry*
Ganglia, Invertebrate / anatomy & histology
Ganglia, Invertebrate / chemistry
Nerve Tissue Proteins / analysis*
Nerve Tissue Proteins / genetics
Peptides / analysis*
Peptides / genetics
Proteomics / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Drosophila Proteins
Nerve Tissue Proteins
Peptides