Variability and recombination of clinical human cytomegalovirus strains from transplantation recipients

J Clin Virol. 2010 Feb;47(2):161-9. doi: 10.1016/j.jcv.2009.11.023.

Abstract

Background: Human cytomegalovirus (HCMV) is the first cause of viral infection in immunocompromised transplanted patients.

Objectives: Here, five HCMV genes were studied to investigate the existence of recombination events in clinical strains ex vivo.

Study design: Sequencing and phylogenetic analysis were conducted on 21 strains from 16 renal and 5 lung transplant recipients.

Results: Nucleotidic polymorphism ranged from 6.6% (US3) to 12% (UL40), with a significant proportion of missense mutations (39-69%), some of which could have a functional impact. Analysis of the concatenated sequence (4804 nucleotides for each strain) evidenced two clusters of sequences presenting a reticulate topology suggestive of recombination events (SplitsTree). Phi-test pointed numerous phylogenetically conflicting signals indicating a high statistical probability of recombination. The subsequent bootscan analysis was consistent with these data.

Conclusions: These results reinforce the prominent role of recombination in HCMV evolutionary history and adaptation to its host.

MeSH terms

  • Adaptation, Biological
  • Adult
  • Cluster Analysis
  • Cytomegalovirus / classification*
  • Cytomegalovirus / genetics*
  • Cytomegalovirus / isolation & purification
  • Cytomegalovirus Infections / virology*
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Evolution, Molecular
  • Female
  • Genotype
  • Humans
  • Immunocompromised Host
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Phylogeny
  • Polymorphism, Genetic*
  • Recombination, Genetic*
  • Sequence Analysis, DNA
  • Transplantation*

Substances

  • DNA, Viral

Associated data

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