Toxicological evaluation of a dietary supplement formulated for male sexual health prior to market release

Regul Toxicol Pharmacol. 2010 Jun;57(1):55-61. doi: 10.1016/j.yrtph.2009.12.006. Epub 2010 Jan 4.

Abstract

The dietary supplement, 112 Degrees, was formulated with the goal of supporting sexual functioning in men. Due to rampant problems with drug adulteration for this category of products, a comprehensive screening for active pharmaceutical agents, with an emphasis on drugs prescribed for erectile dysfunction such as type 5 phosphodiesterase (PDE-5) inhibitors, and known unapproved PDE-5 drug analogues, was performed along with preclinical toxicology studies prior to the introduction of this product into the marketplace. 112 Degrees was found to be free of all pharmaceutical adulterants tested, and was not mutagenic, clastogenic, or genotoxic as demonstrated by the Ames test, chromosomal aberration assay, and mouse micronucleus assay, respectively. The LD(50) in the 14-day acute oral toxicity study was greater than 5000 mg/kg, the highest dose tested.

MeSH terms

  • Animals
  • CHO Cells
  • Consumer Product Safety*
  • Cricetinae
  • Cricetulus
  • Dietary Supplements / toxicity*
  • Dose-Response Relationship, Drug
  • Drug Contamination
  • Drug Evaluation, Preclinical
  • Female
  • Humans
  • Lethal Dose 50
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Micronuclei, Chromosome-Defective / chemically induced
  • Micronucleus Tests
  • Mutation
  • Rats
  • Rats, Sprague-Dawley
  • Salmonella typhimurium / drug effects
  • Salmonella typhimurium / genetics
  • Sexual Behavior / drug effects*
  • Toxicity Tests, Chronic