Abstract
The gene coding for the transacylase subunit (DBT) of the human branched-chain keto acid dehydrogenase complex was localized to chromosome 1 by probing panels of human x mouse chromosome hybrids with an E2 cDNA amplified by the polymerase chain reaction. Additional data with two hybrids containing chromosome 1 fragments suggest that the DBT gene is located on the short arm (1pter----p21) of the chromosome.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
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Acyltransferases / genetics*
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Acyltransferases / metabolism
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Animals
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Base Sequence
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Chromosome Mapping
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Chromosomes, Human, Pair 1*
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DNA Probes
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Humans
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Hybrid Cells
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Ketone Oxidoreductases / genetics*
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Ketone Oxidoreductases / metabolism
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Mice
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Molecular Sequence Data
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Multienzyme Complexes / genetics*
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Multienzyme Complexes / metabolism
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Polymerase Chain Reaction
Substances
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DNA Probes
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Multienzyme Complexes
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Ketone Oxidoreductases
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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
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Acyltransferases
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dihydrolipoamide acyltransferase