Combination of tipifarnib and rapamycin synergistically inhibits the growth of leukemia cells and overcomes resistance to tipifarnib via alteration of cellular signaling pathways

Leuk Res. 2010 Aug;34(8):1057-63. doi: 10.1016/j.leukres.2009.12.011. Epub 2010 Jan 13.

Abstract

Small molecules are attractive agents for the treatment of leukemia. We found that a combination of a farnesyltransferase inhibitor, tipifarnib, and an mTOR inhibitor, rapamycin, synergistically inhibited the growth of myeloid leukemia cell lines and primary leukemia cells by inducing apoptosis and cell-cycle blockage. The combined agents reduced the level of phospho-ERK1/2, suggesting that they altered the network of signaling pathways. They also showed synergistic effects in tipifarnib-resistant K562/RR cells. The results support the utility of this combination as a potential therapy for leukemia. The combination might also be effective in overcoming resistance to tipifarnib.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Combined Chemotherapy Protocols / pharmacology*
  • Apoptosis / drug effects
  • Blotting, Western
  • Cell Cycle / drug effects
  • Cell Proliferation / drug effects
  • Drug Resistance, Neoplasm / drug effects*
  • Flow Cytometry
  • Humans
  • Leukemia / classification
  • Leukemia / drug therapy*
  • Leukemia / pathology
  • Quinolones / administration & dosage
  • Signal Transduction / drug effects*
  • Sirolimus / administration & dosage
  • Tumor Cells, Cultured

Substances

  • Quinolones
  • tipifarnib
  • Sirolimus