Abstract
This study reports the immunomodulatory activity on human monocyte derived dendritic cells (MDDCs) of a vaccine preparation shown to be effective against an HPV16-related tumour in an animal model. The vaccine is composed of extract from Nicotiana benthamiana leaves containing HPV16 E7 protein expressed by a potato virus X-derived vector (NbPVX-E7). The effect of the extract was evaluated on MDDC differentiation and maturation by monitoring the phenotypic expression of specific markers. The results show that NbPVX-E7 does not induce monocyte differentiation to dendritic cells, but does induce MDDC maturation. Plant extract does not influence MDDC-uptake of E7-FITC while it significantly improves the Ovalbumin-FITC uptake, considered as a model antigen. Importantly, NbPVX-E7-pulsed MDDCs/PBMCs are able to prime human blood-derived lymphocytes from healthy individuals to induce HPV16 E7-specific cytotoxic activity. This is a propaedeutic study for a possible use of E7-containing plant extract in human immunotherapy of HPV-related lesions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / isolation & purification
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Adjuvants, Immunologic / pharmacology*
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Antigen Presentation
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Cell Differentiation
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Cell Line, Tumor
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Cell Survival
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Coculture Techniques
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Cytotoxicity, Immunologic
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Dendritic Cells / drug effects
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Dose-Response Relationship, Drug
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Genetic Vectors
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Humans
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Lymphocyte Activation
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Lymphocytes / drug effects
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Lymphocytes / immunology*
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Male
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Nicotiana / genetics
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Nicotiana / metabolism*
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Oncogene Proteins, Viral / biosynthesis
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Oncogene Proteins, Viral / genetics
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Oncogene Proteins, Viral / immunology*
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Oncogene Proteins, Viral / isolation & purification
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Ovalbumin / immunology
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Ovalbumin / metabolism
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Papillomavirus E7 Proteins
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Papillomavirus Vaccines / biosynthesis
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Papillomavirus Vaccines / genetics
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Papillomavirus Vaccines / immunology*
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Papillomavirus Vaccines / isolation & purification
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Plant Extracts / immunology*
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Plant Extracts / isolation & purification
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Plant Leaves
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Plants, Genetically Modified*
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Potexvirus / genetics
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Recombinant Proteins / immunology
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Time Factors
Substances
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Adjuvants, Immunologic
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Oncogene Proteins, Viral
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Papillomavirus E7 Proteins
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Papillomavirus Vaccines
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Plant Extracts
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Recombinant Proteins
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oncogene protein E7, Human papillomavirus type 16
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Ovalbumin