Development of a novel DNA microarray to detect bacterial pathogens in patients with chronic obstructive pulmonary disease (COPD)

Tanya Curran; Wilson A Coulter; Derek J Fairley; Terence McManus; Joe Kidney; Mike Larkin; John E Moore; Peter V Coyle

doi:10.1016/j.mimet.2010.01.004

Development of a novel DNA microarray to detect bacterial pathogens in patients with chronic obstructive pulmonary disease (COPD)

J Microbiol Methods. 2010 Mar;80(3):257-61. doi: 10.1016/j.mimet.2010.01.004. Epub 2010 Jan 14.

Authors

Tanya Curran¹, Wilson A Coulter, Derek J Fairley, Terence McManus, Joe Kidney, Mike Larkin, John E Moore, Peter V Coyle

Affiliation

¹ Microbiology Department, Kelvin Building, The Royal Group of Hospitals and Dental Hospital Health and Social Services Trust, Belfast, BT12 6BA, UK. [email protected]

Abstract

A novel microarray was constructed with DNA PCR product probes targeting species specific functional genes of nine clinically significant respiratory pathogens, including the Gram-positive organisms (Streptococcus pneumoniae, Streptococcus pyogenes), the Gram-negative organisms (Chlamydia pneumoniae, Coxiella burnetii Haemophilus spp., Legionella pneumophila, Moraxella catarrhalis, and Pseudomonas aeruginosa), as well as the atypical bacterium, Mycoplasma pneumoniae. In a "proof-of-concept" evaluation of the developed microarray, the microarray was compared with real-time PCR from 14 sputum specimens from COPD patients. All of the samples positive for bacterial species in real-time PCR were also positive for the same bacterial species using the microarray. This study shows that a microarray using PCR probes is a potentially useful method to monitor the populations of bacteria in respiratory specimens and can be tailored to specific clinical needs such as respiratory infections of particular patient populations, including patients with cystic fibrosis and bronchiectasis.

Publication types

Evaluation Study

MeSH terms

Chlamydophila pneumoniae / genetics
Chlamydophila pneumoniae / isolation & purification
Coxiella burnetii / genetics
Coxiella burnetii / isolation & purification
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Gram-Negative Bacteria / genetics
Gram-Negative Bacteria / isolation & purification*
Gram-Negative Bacterial Infections / diagnosis*
Gram-Negative Bacterial Infections / etiology
Gram-Positive Bacterial Infections / diagnosis*
Gram-Positive Bacterial Infections / etiology
Haemophilus / genetics
Haemophilus / isolation & purification
Humans
Legionella pneumophila / genetics
Legionella pneumophila / isolation & purification
Moraxella catarrhalis / genetics
Moraxella catarrhalis / isolation & purification
Mycoplasma pneumoniae / genetics
Mycoplasma pneumoniae / isolation & purification
Oligonucleotide Array Sequence Analysis / methods*
Pseudomonas aeruginosa / genetics
Pseudomonas aeruginosa / isolation & purification
Pulmonary Disease, Chronic Obstructive / complications
Pulmonary Disease, Chronic Obstructive / microbiology*
Respiratory Tract Infections / diagnosis*
Respiratory Tract Infections / etiology
Sensitivity and Specificity
Species Specificity
Sputum / microbiology
Streptococcus pneumoniae / genetics
Streptococcus pneumoniae / isolation & purification*
Streptococcus pyogenes / genetics
Streptococcus pyogenes / isolation & purification*

Substances

DNA, Bacterial