[Gene identification of mumps wild virus isolated firstly in Jilin province]

Zhongguo Yi Miao He Mian Yi. 2009 Oct;15(5):432-4.
[Article in Chinese]

Abstract

Objective: To observe Cytopathic Effect of wild-type mump viruses in Vero/SLAM cell and identify genotype in a mump outbreak in 2008.

Methods: Throat swabs were collected and to isolated mump virus with Vero/slam cells.Cytopathic effect was observed and determined mump virus type with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Results: Of the total 15 throat swabs, different CPE were observed in 3 throat swabs but genotypes are same, they are F type, 3 throat swabs were not observed cytopathic effect but genotypes were F type.

Conclusion: There were different kinds of cytopathic effect in one mump outbreak although genotypes were same.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Chlorocebus aethiops
  • Cytopathogenic Effect, Viral
  • Disease Outbreaks
  • Female
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Mumps / virology*
  • Mumps virus / classification
  • Mumps virus / genetics*
  • Mumps virus / isolation & purification*
  • Pharynx / virology
  • Vero Cells
  • Young Adult