Abstract
Transgenic Xenopus laevis are commonly used to study gene expression in photoreceptors, but only red rods and red cones are known to exist in the pre-metamorphic stages commonly used in transgenic studies. Using RT-PCR, this study shows that violet cones develop in early pre-metamorphic stages (Stage 35) with the red rods and red cones. Green rod development began in Stage 53 with the onset of metamorphosis.
2010 Wiley-Liss, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Embryo, Nonmammalian / physiology*
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Metamorphosis, Biological*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Retinal Cone Photoreceptor Cells / cytology*
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Retinal Cone Photoreceptor Cells / metabolism
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Retinal Rod Photoreceptor Cells / cytology*
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Retinal Rod Photoreceptor Cells / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Rod Opsins / genetics
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Rod Opsins / metabolism
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Xenopus laevis / embryology*
Substances
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RNA, Messenger
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Rod Opsins