Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI

Vanesa Y Rawe; Heydy Uriondo Boudri; Cristian Alvarez Sedó; Martina Carro; Sergio Papier; Florencia Nodar

doi:10.1016/j.rbmo.2009.12.004

Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI

Reprod Biomed Online. 2010 Mar;20(3):320-3. doi: 10.1016/j.rbmo.2009.12.004. Epub 2009 Dec 11.

Authors

Vanesa Y Rawe¹, Heydy Uriondo Boudri, Cristian Alvarez Sedó, Martina Carro, Sergio Papier, Florencia Nodar

Affiliation

¹ Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina. [email protected]

PMID: 20096634
DOI: 10.1016/j.rbmo.2009.12.004

Abstract

Magnetic activated cell sorting (MACS) with annexin V microbeads recognizes externalized phosphatidylserine (PS) residues on the surface of apoptotic spermatozoa. The successful use of this novel technique applied to a highly apoptotic semen sample before performing intracytoplasmic sperm injection (ICSI) is reported here. The use of annexin V microbeads for selecting non-apoptotic spermatozoa seems to reduce the percentage of altered cells, improving the chance of pregnancy after ICSI.

Publication types

Case Reports

MeSH terms

Adult
Annexin A5
Cell Separation / methods*
DNA Fragmentation*
Female
Humans
In Situ Nick-End Labeling
Infant, Newborn
Infertility, Male / therapy*
Magnetics
Male
Microspheres
Pregnancy
Pregnancy Outcome
Sperm Injections, Intracytoplasmic / methods*

Substances

Annexin A5