Generation of monoclonal antibodies against human regulatory T cells

J Immunol Methods. 2010 Feb 28;353(1-2):62-70. doi: 10.1016/j.jim.2010.01.002. Epub 2010 Jan 22.

Abstract

Natural CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers and to identify targets for a therapeutic modulation of Tregs. Here we present a methodology optimized to efficiently induce and select mAb against human Tregs by repeated immunization of mice with Tregs from a single donor and a differential two-step flow cytometry-based hybridoma screening procedure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / isolation & purification
  • Antibody Specificity
  • Cell Separation / methods
  • Epitope Mapping
  • Epitopes
  • Female
  • Flow Cytometry
  • Forkhead Transcription Factors / analysis
  • Humans
  • Hybridomas
  • Immunization Schedule
  • Interleukin-2 Receptor alpha Subunit / analysis
  • Leukapheresis
  • Mice
  • Receptors, Antigen, T-Cell / immunology*
  • T-Lymphocytes, Regulatory / immunology*

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • IL2RA protein, human
  • Interleukin-2 Receptor alpha Subunit
  • Receptors, Antigen, T-Cell