TGF-beta activity protects against inflammatory aortic aneurysm progression and complications in angiotensin II-infused mice

J Clin Invest. 2010 Feb;120(2):422-32. doi: 10.1172/JCI38136. Epub 2010 Jan 25.

Abstract

Complicated abdominal aortic aneurysm (AAA) is a major cause of mortality in elderly men. Ang II-dependent TGF-beta activity promotes aortic aneurysm progression in experimental Marfan syndrome. However, the role of TGF-beta in experimental models of AAA has not been comprehensively assessed. Here, we show that systemic neutralization of TGF-beta activity breaks the resistance of normocholesterolemic C57BL/6 mice to Ang II-induced AAA formation and markedly increases their susceptibility to the disease. These aneurysms displayed a large spectrum of complications on echography, including fissuration, double channel formation, and rupture, leading to death from aneurysm complications. The disease was refractory to inhibition of IFN-gamma, IL-4, IL-6, or TNF-alpha signaling. Genetic deletion of T and B cells or inhibition of the CX3CR1 pathway resulted in partial protection. Interestingly, neutralization of TGF-beta activity enhanced monocyte invasiveness, and monocyte depletion markedly inhibited aneurysm progression and complications. Finally, TGF-beta neutralization increased MMP-12 activity, and MMP-12 deficiency prevented aneurysm rupture. These results clearly identify a critical role for TGF-beta in the taming of the innate immune response and the preservation of vessel integrity in C57BL/6 mice, which contrasts with its reported pathogenic role in Marfan syndrome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / metabolism
  • Angiotensin II / toxicity*
  • Animals
  • Anti-Inflammatory Agents / therapeutic use
  • Aortic Aneurysm, Abdominal / diagnostic imaging
  • Aortic Aneurysm, Abdominal / drug therapy*
  • Aortic Aneurysm, Abdominal / pathology
  • Aortic Aneurysm, Abdominal / prevention & control
  • Disease Progression
  • Inflammation / immunology
  • Inflammation / physiopathology*
  • Inflammation / prevention & control
  • Male
  • Matrix Metalloproteinase 12 / deficiency
  • Matrix Metalloproteinase 2 / deficiency
  • Mice
  • Mice, Inbred C57BL
  • Transforming Growth Factor beta / antagonists & inhibitors
  • Transforming Growth Factor beta / immunology
  • Transforming Growth Factor beta / pharmacology*
  • Ultrasonography

Substances

  • Anti-Inflammatory Agents
  • Transforming Growth Factor beta
  • Angiotensin II
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 12