CBFA2T3-ZNF651, like CBFA2T3-ZNF652, functions as a transcriptional corepressor complex

FEBS Lett. 2010 Mar 5;584(5):859-64. doi: 10.1016/j.febslet.2010.01.047. Epub 2010 Jan 30.

Abstract

A significant proportion of the human genome codes for transcription factors. Balanced activity of transcriptional activators and repressors is essential for normal development and differentiation. Previously we reported that a classical C2H2 zinc finger DNA binding protein ZNF652 functionally interacts with CBFA2T3 to repress transcription of genes containing ZNF652 consensus DNA binding sequence within the promoters of these target genes. Here we show that ZNF651 is a ZNF652 paralogue that shares a common DNA binding sequence with ZNF652 and represses target gene expression through the formation of a CBFA2T3-ZNF651 corepressor complex. It is suggested that CBFA2T3-ZNF651 and CBFA2T3-ZNF652 repressor complexes perform functionally similar roles in a tissue-specific manner.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Western
  • Cell Line
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Electrophoretic Mobility Shift Assay
  • HeLa Cells
  • Humans
  • Immunoprecipitation
  • Molecular Sequence Data
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Protein Binding
  • Repressor Proteins / chemistry
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Tumor Suppressor Proteins / chemistry
  • Tumor Suppressor Proteins / genetics
  • Tumor Suppressor Proteins / metabolism*

Substances

  • CBFA2T3 protein, human
  • DNA-Binding Proteins
  • Phosphoproteins
  • Repressor Proteins
  • Tumor Suppressor Proteins
  • ZNF652 protein, human