Direct detection of methicillin resistance in Staphylococcus aureus in blood culture broth by use of a penicillin binding protein 2a latex agglutination test

Qinfang Qian; Lata Venkataraman; James E Kirby; Howard S Gold; Toshiaki Yamazumi

doi:10.1128/JCM.01517-09

Direct detection of methicillin resistance in Staphylococcus aureus in blood culture broth by use of a penicillin binding protein 2a latex agglutination test

J Clin Microbiol. 2010 Apr;48(4):1420-1. doi: 10.1128/JCM.01517-09. Epub 2010 Feb 3.

Authors

Qinfang Qian¹, Lata Venkataraman, James E Kirby, Howard S Gold, Toshiaki Yamazumi

Affiliation

¹ Division of Laboratory and Transfusion Medicine, Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA. [email protected]

Abstract

We studied the utility of performing a penicillin binding protein 2a latex agglutination (PBP-LA) assay directly on Bactec blood culture broth samples containing Staphylococcus aureus to rapidly detect methicillin resistance. The sensitivity, specificity, positive predictive value, and negative predictive value of this method were 94.1%, 97.5%, 98%, and 92.9%, respectively.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / analysis*
Bacterial Proteins / immunology
Bacteriological Techniques / methods*
Blood / microbiology*
Humans
Latex Fixation Tests / methods
Methicillin-Resistant Staphylococcus aureus / chemistry*
Methicillin-Resistant Staphylococcus aureus / isolation & purification*
Penicillin-Binding Proteins / analysis*
Penicillin-Binding Proteins / immunology
Predictive Value of Tests
Sensitivity and Specificity

Substances

Bacterial Proteins
Penicillin-Binding Proteins