Abstract
A/Hong Kong/213/97 (HK213; H5N1), isolated from a human, binds to both avian- and human-type receptors, due to a haemagglutinin (HA) mutation probably acquired during adaptation to humans. Duck passage of this virus conferred lethality in ducks. Sequence analyses of the duck-passaged virus revealed that its HA gene reverted back to one recognizing only avian-type receptors, and consequently it bound human tissue to a lesser extent. This finding suggests that viruses with human-type receptor specificity are unlikely to be maintained in waterfowl, unlike those with the human-type PB2 mutation, such as H5N1 viruses of the Qinghai Lake lineage.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Ducks
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Hemagglutinin Glycoproteins, Influenza Virus / genetics*
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Hemagglutinin Glycoproteins, Influenza Virus / metabolism*
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Humans
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Influenza A Virus, H5N1 Subtype / genetics
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Influenza A Virus, H5N1 Subtype / isolation & purification
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Influenza A Virus, H5N1 Subtype / pathogenicity*
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Influenza in Birds / virology*
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Influenza, Human / virology*
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Molecular Sequence Data
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Mutation, Missense*
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Poultry Diseases / virology
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RNA, Viral / genetics
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Sequence Analysis, DNA
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Serial Passage
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Survival Analysis
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Virus Attachment*
Substances
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Hemagglutinin Glycoproteins, Influenza Virus
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RNA, Viral
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hemagglutinin, avian influenza A virus
Associated data
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GENBANK/AB557744
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GENBANK/AB557745
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GENBANK/AB557746
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GENBANK/AB557747
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GENBANK/AB557748
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GENBANK/AB557749
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GENBANK/AB557750