Lactoferrin protects against concanavalin A-induced liver injury in mice

Liver Int. 2010 Apr;30(4):623-32. doi: 10.1111/j.1478-3231.2009.02199.x. Epub 2010 Jan 29.

Abstract

Background: Liver diseases, caused by viral infection, autoimmune conditions, alcohol ingestion or the use of certain drugs, are a significant health issue, as many can develop into liver failure. Lactoferrin (Lac) is an iron-binding glycoprotein that belongs to the transferrin family. Owing to its multiple biological functions, Lac has been evaluated in a number of clinical trials to treat infections, inflammation and cancer.

Aim: The present study aims to reveal a profound hepatoprotective effect of Lac, using a mouse model of Concanavalin A (Con A)-induced hepatitis, which mimics the pathophysiology of human viral and autoimmune hepatitis.

Method: C57Bl/6J mice were injected with bovine Lac following Con A challenge. The effects of Lac on interferon (IFN)-gamma and interleukin (IL)-4 expression were determined. The roles of Lac on T-cell apoptosis and activation, and leukocytes infiltration were examined.

Result: The data demonstrated that the protective effect of Lac was attributed to its ability to inhibit T-cell activation and production of IFN-gamma, as well as to suppress IL-4 production by hepatic natural killer T cells.

Conclusion: These findings indicate a great therapeutic potential of Lac in treating in treating inflammatory hepatitis and possibly other inflammatory diseases.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cattle
  • Chemical and Drug Induced Liver Injury / metabolism
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Concanavalin A
  • Cytokines / metabolism*
  • Disease Models, Animal
  • Flow Cytometry
  • Hepatitis / pathology
  • Hepatitis / prevention & control*
  • Immunohistochemistry
  • Interferons
  • Interleukin-4 / metabolism
  • Lactoferrin / pharmacology*
  • Liver / drug effects*
  • Liver / metabolism
  • Liver / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Probability
  • Random Allocation
  • Th1 Cells / cytology
  • Th1 Cells / drug effects

Substances

  • Cytokines
  • Concanavalin A
  • Interleukin-4
  • Interferons
  • Lactoferrin