Pathway of ATP utilization and duplex rRNA unwinding by the DEAD-box helicase, DbpA

Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4046-50. doi: 10.1073/pnas.0913081107. Epub 2010 Feb 16.

Abstract

DEAD-box RNA helicase proteins use the energy of ATP hydrolysis to drive the unwinding of duplex RNA. However, the mechanism that couples ATP utilization to duplex RNA unwinding is unknown. We measured ATP utilization and duplex RNA unwinding by DbpA, a non-processive bacterial DEAD-box RNA helicase specifically activated by the peptidyl transferase center (PTC) of 23S rRNA. Consumption of a single ATP molecule is sufficient to unwind and displace an 8 base pair rRNA strand annealed to a 32 base pair PTC-RNA "mother strand" fragment. Strand displacement occurs after ATP binding and hydrolysis but before P(i) product release. P(i) release weakens binding to rRNA, thereby facilitating the release of the unwound rRNA mother strand and the recycling of DbpA for additional rounds of unwinding. This work explains how ATPase activity of DEAD-box helicases is linked to RNA unwinding.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenosine Diphosphate / metabolism
  • Adenosine Triphosphate / metabolism*
  • DEAD-box RNA Helicases / metabolism*
  • Escherichia coli Proteins / metabolism*
  • Hydrolysis
  • Nucleic Acid Denaturation
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / metabolism*
  • Recombinant Proteins / metabolism

Substances

  • Escherichia coli Proteins
  • RNA, Ribosomal
  • Recombinant Proteins
  • Adenosine Diphosphate
  • Adenosine Triphosphate
  • dbpA protein, E coli
  • DEAD-box RNA Helicases