Ultra-fast HPLC-ICP-MS analysis of oxaliplatin in patient urine

Anal Bioanal Chem. 2010 May;397(1):401-406. doi: 10.1007/s00216-010-3504-3. Epub 2010 Feb 19.

Abstract

A novel method for rapid HPLC-ICP-MS analysis of oxaliplatin in human urine was developed implementing a stationary HPLC phase with a particle size of 1.8 microm. The method allowed a cycle time of <1 min at a HPLC flow rate of 0.9 mL min(-1). Procedural limits of detection of 0.05 microg L(-1) oxaliplatin (150 fg on column) were obtained. Analysis of oxaliplatin in patient urine showed that accurate quantification of the intact drug demanded for storage at -80 degrees C and rapid measurement after thawing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / urine*
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • Mass Spectrometry / methods*
  • Organoplatinum Compounds / urine*
  • Oxaliplatin

Substances

  • Antineoplastic Agents
  • Organoplatinum Compounds
  • Oxaliplatin