Compensatory hypertrophy induced by ventricular cardiomyocyte-specific COX-2 expression in mice

J Mol Cell Cardiol. 2010 Jul;49(1):88-94. doi: 10.1016/j.yjmcc.2010.01.021. Epub 2010 Feb 17.

Abstract

Cyclooxygenase-2 (COX-2) is an important mediator of inflammation in stress and disease states. Recent attention has focused on the role of COX-2 in human heart failure and diseases owing to the finding that highly specific COX-2 inhibitors (i.e., Vioxx) increased the risk of myocardial infarction and stroke in chronic users. However, the specific impact of COX-2 expression in the intact heart remains to be determined. We report here the development of a transgenic mouse model, using a loxP-Cre approach, which displays robust COX-2 overexpression and subsequent prostaglandin synthesis specifically in ventricular myocytes. Histological, functional, and molecular analyses showed that ventricular myocyte specific COX-2 overexpression led to cardiac hypertrophy and fetal gene marker activation, but with preserved cardiac function. Therefore, specific induction of COX-2 and prostaglandin in vivo is sufficient to induce compensated hypertrophy and molecular remodeling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cardiomegaly / metabolism
  • Cardiomegaly / pathology
  • Cyclooxygenase 2 / metabolism
  • Cyclooxygenase 2 Inhibitors / metabolism
  • Heart Failure / metabolism
  • Heart Failure / pathology
  • Heart Failure / physiopathology
  • Heart Ventricles / pathology
  • Heart Ventricles / physiopathology*
  • Hypertrophy / metabolism
  • Hypertrophy / pathology
  • Hypertrophy / physiopathology
  • Lactones
  • Mice
  • Mice, Transgenic
  • Myocardial Infarction / metabolism
  • Myocardial Infarction / pathology
  • Myocardial Infarction / physiopathology
  • Myocytes, Cardiac / metabolism*
  • Myocytes, Cardiac / pathology
  • Sulfones

Substances

  • Cyclooxygenase 2 Inhibitors
  • Lactones
  • Sulfones
  • rofecoxib
  • Cyclooxygenase 2
  • PTGS2 protein, human