Plant-specific multisubunit RNA polymerase in gene silencing

Epigenetics. 2010 Jan 1;5(1):4-8. doi: 10.4161/epi.5.1.10435. Epub 2010 Jan 26.

Abstract

In recent years, a major breakthrough in the study of epigenetic silencing in eukaryotes came with the discovery that the RNA-interference pathway (RNAi) is generally implicated in heterochromatin assembly and gene silencing. An important and paradoxical feature of the RNAi-mediated heterochromatin pathways is their requirement for some form of transcription. In fission yeast, Schizosaccharomyces pombe, centromeric siRNAs have been shown to derive from chromatin-bound nascent transcripts produced by RNA polymerase II (PolII) at the site of heterochromatin formation. Likewise, chromatin-bound nascent transcripts generated by a PolII-related DNA-dependent RNA polymerase, known as PolIVb/PolV, have recently been implicated in RNA-directed DNA methylation (RdDM), the prominent RNAi-mediated chromatin pathway in plants. In this review we discuss recent work on the plant-specific PolII variant enzymes and discuss the mechanistic convergences that have been observed in the role of these enzymes in their respective siRNA-mediated heterochromatin formation pathways.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Arabidopsis / genetics*
  • Centromere / ultrastructure
  • Chromatin / metabolism
  • DNA Methylation
  • DNA-Directed RNA Polymerases / chemistry*
  • DNA-Directed RNA Polymerases / genetics*
  • Gene Silencing*
  • Heterochromatin / chemistry
  • Heterochromatin / metabolism
  • Models, Biological
  • Plants / enzymology*
  • Plants / genetics*
  • RNA Interference*
  • RNA Polymerase II / metabolism
  • RNA, Small Interfering / metabolism
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces pombe Proteins / metabolism
  • Transcription, Genetic

Substances

  • Chromatin
  • Heterochromatin
  • RNA, Small Interfering
  • Schizosaccharomyces pombe Proteins
  • RNA Polymerase II
  • DNA-Directed RNA Polymerases