Abstract
The in vivo-mimic assay system using silkworm larvae was used as a screening tool to discover antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Microbial culture broths were screened in this in vivo-mimic assay system and a culture broth of Streptomyces sp. K04-0144 was selected. New antibiotics, designated nosokomycins A-D, were isolated from the culture broth by HP-20 and ODS column chromatography and HPLC. Nosokomycins inhibited the growth of MRSA with MIC values of 0.125 microg ml(-1) using the liquid microdilution method. Furthermore, MRSA-infected silkworms survived when nosokomycin A or B was injected at a dose of 50 microg per larva.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / biosynthesis
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology*
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Bombyx
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Carbohydrate Sequence
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Fermentation
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Larva
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Methicillin-Resistant Staphylococcus aureus / drug effects*
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Methicillin-Resistant Staphylococcus aureus / growth & development
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Microbial Sensitivity Tests / methods
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Molecular Sequence Data
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Oligosaccharides / biosynthesis
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Oligosaccharides / isolation & purification*
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Oligosaccharides / pharmacology*
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Sesquiterpenes / isolation & purification
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Sesquiterpenes / metabolism
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Sesquiterpenes / pharmacology
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Staphylococcal Infections / drug therapy*
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Streptomyces / chemistry*
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Streptomyces / metabolism
Substances
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Anti-Bacterial Agents
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Oligosaccharides
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Sesquiterpenes