Abstract
The preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized in TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bambermycins / pharmacology
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Enzyme Inhibitors / pharmacology
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Fluorescence*
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Glycosyltransferases / antagonists & inhibitors
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Glycosyltransferases / metabolism*
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Molecular Structure
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Polyisoprenyl Phosphates / chemical synthesis*
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Polyisoprenyl Phosphates / chemistry
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Polyisoprenyl Phosphates / metabolism*
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Structure-Activity Relationship
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Substrate Specificity
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Uridine Diphosphate N-Acetylmuramic Acid / analogs & derivatives*
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Uridine Diphosphate N-Acetylmuramic Acid / chemistry
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Uridine Diphosphate N-Acetylmuramic Acid / metabolism
Substances
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Enzyme Inhibitors
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Polyisoprenyl Phosphates
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Uridine Diphosphate N-Acetylmuramic Acid
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muramyl-NAc-(pentapeptide)pyrophosphoryl-undecaprenol
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Bambermycins
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moenomycin A
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Glycosyltransferases