Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination

Vaccine. 2010 Feb 23;28(8):2020-5. doi: 10.1016/j.vaccine.2009.10.062.

Abstract

Mycobacterial DNA-binding protein 1 (MDP1) is a major protein antigen in mycobacteria and induces protective immunity against Mycobacterium tuberculosis infection in mice. In this study we determined murine T-cell epitopes on MDP1 with MDP1 DNA immunization in mice. We analyzed interferon-gamma production from the MDP1 DNA-immune splenocytes in response to 20-mer overlapping peptides covering MDP1 protein. We identified several CD4+ T-cell epitopes in three inbred mouse strains and one CD8+ T-cell epitope in C57BL/6 mice. These T-cell epitopes would be feasible for analysis of the role of MDP1-specific T-cells in protective immunity and for future vaccine design against M. tuberculosis infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Amino Acid Sequence
  • Animals
  • Antigens, Bacterial / immunology*
  • Bacterial Proteins / immunology*
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Cells, Cultured
  • DNA-Binding Proteins / immunology*
  • Epitopes, T-Lymphocyte / immunology*
  • Female
  • Interferon-gamma / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Plasmids
  • Spleen / cytology
  • Spleen / immunology
  • Tuberculosis Vaccines / immunology*
  • Vaccines, DNA / immunology*

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • DNA-Binding Proteins
  • Epitopes, T-Lymphocyte
  • MDP1 protein, Mycobacterium
  • Tuberculosis Vaccines
  • Vaccines, DNA
  • Interferon-gamma