Novel method to detect a construct-specific sequence of the acetolactate synthase gene in genetically-modified flax CDC Triffid (FP967)

Biol Pharm Bull. 2010;33(3):532-4. doi: 10.1248/bpb.33.532.

Abstract

During the fall of 2009, a trace of unauthorized genetically modified (GM) flax (Linum usitatissimum L.) line, CDC Triffid, which is resistant to sulfonylurea herbicides, was detected in many countries including Japan. A method to reliably identify the CDC Triffid line was urgently required. We developed a novel construct-specific real-time polymerase chain reaction (PCR) method to identify the mutant acetolactate synthase gene in the CDC Triffid line. We confirmed that the method can detect 0.001% GM flax in DNA mixing solution. The study shows that the developed method is specific, sensitive and reliable way to monitor a trace of CDC Triffid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetolactate Synthase / genetics*
  • Base Sequence
  • DNA, Plant
  • Flax / genetics*
  • Genes, Plant*
  • Mutation*
  • Plants, Genetically Modified*
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA / methods*

Substances

  • DNA, Plant
  • Acetolactate Synthase