CRP-induced levels of oxidative stress are higher in brain than aortic endothelial cells

Dorothea Closhen; Bianca Bender; Heiko J Luhmann; Christoph R W Kuhlmann

doi:10.1016/j.cyto.2010.02.011

CRP-induced levels of oxidative stress are higher in brain than aortic endothelial cells

Cytokine. 2010 May;50(2):117-20. doi: 10.1016/j.cyto.2010.02.011. Epub 2010 Mar 5.

Authors

Dorothea Closhen¹, Bianca Bender, Heiko J Luhmann, Christoph R W Kuhlmann

Affiliation

¹ University Medical Center of the Johannes Gutenberg University Mainz, Institute of Physiology and Pathophysiology, Duesbergweg 6, Mainz, Germany.

PMID: 20207160
DOI: 10.1016/j.cyto.2010.02.011

Abstract

C-reactive protein (CRP) has been demonstrated to induce blood-brain barrier disruption (BBB) involving NAD(P)H-oxidase dependent oxidative stress. It is unclear why CRP affects the BBB and not other vascular beds following stroke. Therefore we examined CRP receptor and NAD(P)H-oxidase expression levels in bovine brain- (BEC) and aortic endothelial cells. Dichlorodihydrofluorescein measurements revealed significantly higher CRP-induced reactive oxygen species (ROS) levels in BEC. Protein expression of the CRP-receptors CD16, CD32 and of the NAD(P)H-oxidase subunit p22phox were also significantly higher in BEC. In conclusion BEC show a higher vulnerability to CRP due to increased levels of CRP receptors and the NAD(P)H-oxidase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aorta / cytology*
Brain / cytology*
C-Reactive Protein / pharmacology*
Cattle
Endothelial Cells / drug effects*
Endothelial Cells / enzymology
Endothelial Cells / metabolism*
NADPH Oxidases / metabolism
Oxidative Stress / drug effects*
Receptors, IgG / metabolism

Substances

Receptors, IgG
C-Reactive Protein
NADPH Oxidases