Evaluation of seven X-chromosomal short tandem repeat loci located within the Xq26 region

Heike Rodig; Frank Kloep; Lydia Weissbach; Christa Augustin; Jeannett Edelmann; Sandra Hering; Reinhard Szibor; Frank Götz; Werner Brabetz

doi:10.1016/j.fsigen.2009.08.010

Evaluation of seven X-chromosomal short tandem repeat loci located within the Xq26 region

Forensic Sci Int Genet. 2010 Apr;4(3):194-9. doi: 10.1016/j.fsigen.2009.08.010. Epub 2009 Sep 18.

Authors

Heike Rodig¹, Frank Kloep, Lydia Weissbach, Christa Augustin, Jeannett Edelmann, Sandra Hering, Reinhard Szibor, Frank Götz, Werner Brabetz

Affiliation

¹ Biotype AG, Moritzburger Weg 67, 01109 Dresden, Germany.

PMID: 20215031
DOI: 10.1016/j.fsigen.2009.08.010

Abstract

In this study a set of 29 X-chromosomal short tandem repeats (STRs) located within the Xq26 region was evaluated. These STRs were found within the 133.14-133.45Mb region around the HPRTB locus. Evaluation of the microsatellites was performed with regard to polymorphism, reliable amplification, and low stutter artefacts. DXS10101, DXS10102, and DXS10103 were identified as those X-STRs with highest diversity; i.e. PIC values of 0.7174-0.8933. The locus DXS10101 was the optimal candidate for the integration in the commercial available test system Mentype Argus X-8 PCR amplification kit.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Human, X*
DNA Primers
Electrophoresis
Haplotypes
Humans
Polymerase Chain Reaction
Polymorphism, Genetic
Sequence Analysis, DNA
Tandem Repeat Sequences*

Substances

DNA Primers