Abstract
The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.
MeSH terms
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Amino Acid Substitution / genetics
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Antiviral Agents / pharmacology
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Drug Resistance, Viral*
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Humans
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Influenza A Virus, H1N1 Subtype / drug effects*
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Influenza A Virus, H1N1 Subtype / genetics
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Influenza A Virus, H1N1 Subtype / isolation & purification
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Influenza, Human / virology*
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Molecular Sequence Data
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Mutation, Missense*
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Neuraminidase / genetics*
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Oseltamivir / pharmacology
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sequence Analysis, DNA
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Time Factors
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Viral Proteins / genetics*
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Virology / methods*
Substances
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Antiviral Agents
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RNA, Viral
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Viral Proteins
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Oseltamivir
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NA protein, influenza A virus
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Neuraminidase