Abstract
Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-beta-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA(11), and 2 novel IS elements. The cfiA(11) gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.
2010 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution / genetics
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / genetics*
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Bacteroides Infections / microbiology
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Bacteroides fragilis / drug effects*
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Bacteroides fragilis / genetics*
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Bacteroides fragilis / isolation & purification
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Carbapenems / pharmacology*
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DNA Transposable Elements*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Humans
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Molecular Sequence Data
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Republic of Korea
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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beta-Lactam Resistance*
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beta-Lactamases / biosynthesis
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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DNA Transposable Elements
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DNA, Bacterial
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beta-Lactamases
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carbapenemase
Associated data
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GENBANK/AY372695
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GENBANK/AY372696