Novel Burkholderiales 23S rRNA genes identified in ileal biopsy samples from children: preliminary evidence that a subtype is associated with perianal Crohn's disease

J Clin Microbiol. 2010 May;48(5):1939-42. doi: 10.1128/JCM.02261-09. Epub 2010 Mar 17.

Abstract

A novel family of Burkholderiales bacteria was identified in ileal biopsy specimens from children presenting with symptoms of inflammatory bowel disease. A molecular subtyping approach based on sequencing of a variable region of the bacteria's 23S rRNA genes identified three variants. Pilot analysis identified one variant to be significantly associated with perianal Crohn's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Biopsy
  • Burkholderia / classification*
  • Burkholderia / genetics*
  • Burkholderia / isolation & purification
  • Child
  • Child, Preschool
  • Cluster Analysis
  • Crohn Disease / microbiology*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Humans
  • Ileum / microbiology*
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 23S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 23S

Associated data

  • GENBANK/GU189522
  • GENBANK/GU189523
  • GENBANK/GU189524
  • GENBANK/GU189525
  • GENBANK/GU189526
  • GENBANK/GU189527
  • GENBANK/GU189528
  • GENBANK/GU189529
  • GENBANK/GU189530
  • GENBANK/GU189531