Phylogenetic and biological characterization of Newcastle disease virus isolates from Pakistan

J Clin Microbiol. 2010 May;48(5):1892-4. doi: 10.1128/JCM.00148-10. Epub 2010 Mar 17.

Abstract

Eight Newcastle disease virus isolates from Pakistan were sequenced and characterized. A PCR matrix gene assay, designed to detect all avian paramyxovirus 1, did not detect four of the isolates. A new matrix gene test that detected all isolates was developed. Phylogenetic analysis and pathotyping confirmed that virulent viruses of different genotypes are circulating in Pakistan.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Chickens
  • Cluster Analysis
  • Genotype
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Newcastle Disease / epidemiology*
  • Newcastle Disease / virology*
  • Newcastle disease virus / genetics*
  • Newcastle disease virus / isolation & purification
  • Newcastle disease virus / pathogenicity*
  • Pakistan / epidemiology
  • Phylogeny
  • Poultry Diseases / epidemiology*
  • Poultry Diseases / virology*
  • RNA, Viral / genetics
  • Sequence Analysis, DNA
  • Sequence Homology

Substances

  • RNA, Viral

Associated data

  • GENBANK/GU182322
  • GENBANK/GU182323
  • GENBANK/GU182324
  • GENBANK/GU182325
  • GENBANK/GU182326
  • GENBANK/GU182327
  • GENBANK/GU182328
  • GENBANK/GU182329
  • GENBANK/GU182330
  • GENBANK/GU182331
  • GENBANK/GU182332
  • GENBANK/GU182333
  • GENBANK/GU182334
  • GENBANK/GU182335