Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry

Anal Biochem. 2010 Jul 15;402(2):191-3. doi: 10.1016/j.ab.2010.03.033. Epub 2010 Mar 31.

Abstract

Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error=6%), precise (intra- and interday imprecision <8%), and sensitive (limit of detection=0.06pmol). Allantoin levels measured in control samples were comparable to literature values.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Allantoin / urine*
  • Child
  • Child, Preschool
  • Chromatography, High Pressure Liquid / methods
  • Female
  • Humans
  • Limit of Detection
  • Male
  • Middle Aged
  • Tandem Mass Spectrometry / economics
  • Tandem Mass Spectrometry / methods*
  • Young Adult

Substances

  • Allantoin