Abstract
The methanolic extract of the bark of Betula platyphylla was found to suppress antigen mediated degranulation of RBL-2H3 cells. Four arylbutanoids (1-4) and eight diarylhepatonoids (5-12) were isolated from the methanolic extract using bioassay-guided fractionation. Among them, compounds 4 and 12 were isolated and assigned for the first time. Compounds 1, 2, 3, 5, 10, and 12 showed remarkable inhibitory activity against the degranulation of RBL-2H3 by antigen stimulation in a dose dependent manner at the concentrations ranging from 10 microM to 100 microM.
2010 Elsevier Ltd. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anti-Inflammatory Agents / chemistry*
-
Anti-Inflammatory Agents / isolation & purification
-
Anti-Inflammatory Agents / pharmacology
-
Antigens / immunology
-
Betula / chemistry*
-
Cell Degranulation / drug effects*
-
Cell Line, Tumor
-
Glucosides / chemistry*
-
Glucosides / isolation & purification
-
Glucosides / pharmacology
-
Meglutol / analogs & derivatives*
-
Meglutol / chemistry
-
Meglutol / isolation & purification
-
Meglutol / pharmacology
-
Phenols / chemistry*
-
Phenols / isolation & purification
-
Phenols / pharmacology
-
Plant Bark / chemistry
-
Rats
-
Trisaccharides / chemistry*
-
Trisaccharides / isolation & purification
-
Trisaccharides / pharmacology
-
beta-N-Acetylhexosaminidases / metabolism
Substances
-
Anti-Inflammatory Agents
-
Antigens
-
Glucosides
-
Phenols
-
Trisaccharides
-
Meglutol
-
beta-N-Acetylhexosaminidases