Expressions of SE-1, CD31 and CD105 in the vascular endothelial cells and serum of rat with hepatocellular carcinoma

Chin Med J (Engl). 2010 Mar 20;123(6):730-3.

Abstract

Background: Hepatocellular carcinoma (HCC) is one of the deadliest cancers worldwide. In order to investigate the molecular biologic mechanism of HCC's development, we studied the expressions of SE-1, CD105 and CD31 in tumor endothelial cells (TECs) of HCC and in the serum of rats.

Methods: We analyzed the expressions of SE-1, CD31 and CD105 in rat HCC tumor tissues using immunohistochemistry (IHC). Twenty HCC bearing rats and eighteen normal rats were examined for the expressions of SE-1, CD31 and CD105 antigens in serum by enzyme-linked immunosorbent assay (ELISA).

Results: SE-1, CD31 and CD105 antigens were detected both in HCC tissue and in normal liver tissue with higher expressions of CD31 and CD105 in HCC while the SE-1 antigen expression was higher in normal liver. Similarly, serum CD31 and CD105 in rats with HCC were significantly increased compared with normal rats (t = 2.8628, P = 0.0086; t = 4.4922, P < 0.0001, respectively). In contrast, SE-1 antigen in HCC rat serum was significantly decreased compared with normal rats (t = 3.4983, P = 0.0011).

Conclusion: SE-1, CD31 and CD105 are closely related with liver tumor angiogenesis, which is similar to their performances in terms of their expressions in the serum.

MeSH terms

  • Animals
  • Antigens, CD / blood*
  • Carcinoma, Hepatocellular / blood supply
  • Carcinoma, Hepatocellular / chemistry*
  • Endothelial Cells / chemistry*
  • Endothelial Cells / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Immunohistochemistry
  • Liver Neoplasms, Experimental / blood supply
  • Liver Neoplasms, Experimental / chemistry*
  • Male
  • Neovascularization, Pathologic / blood
  • Platelet Endothelial Cell Adhesion Molecule-1 / blood*
  • Rats
  • Rats, Inbred BUF

Substances

  • Antigens, CD
  • Platelet Endothelial Cell Adhesion Molecule-1