Asna1/TRC40-mediated membrane insertion of tail-anchored proteins

J Cell Sci. 2010 May 1;123(Pt 9):1522-30. doi: 10.1242/jcs.055970. Epub 2010 Apr 7.

Abstract

Tail-anchored (TA) proteins insert post-translationally into the membrane of the endoplasmic reticulum (ER) and span the membrane by their C-terminal transmembrane domain. We have reconstituted membrane insertion of TA proteins from recombinant Asna1/TA protein complexes and ER-derived membranes. Our data show that Asna1 can mediate membrane insertion of RAMP4 and Sec61beta without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane. By contrast, membrane insertion of cytochrome b5 can proceed independently of Asna1 and nucleotides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arsenite Transporting ATPases / isolation & purification
  • Arsenite Transporting ATPases / metabolism*
  • Chromatography, Gel
  • Cytochromes b5 / metabolism
  • Humans
  • Intracellular Membranes / metabolism*
  • Membrane Proteins / metabolism*
  • Microsomes / metabolism
  • Molecular Weight
  • Nucleotides / metabolism
  • Rabbits
  • Recombinant Fusion Proteins / metabolism
  • SEC Translocation Channels
  • Solubility

Substances

  • GET3 protein, human
  • Membrane Proteins
  • Nucleotides
  • Recombinant Fusion Proteins
  • SEC Translocation Channels
  • Cytochromes b5
  • Arsenite Transporting ATPases