Divergent effects on phagocytosis by macrophage-derived oxygen radicals

J Innate Immun. 2009;1(6):592-8. doi: 10.1159/000235583. Epub 2009 Aug 14.

Abstract

Clearance of senescent cells from damaged tissue is a crucial step to successfully resolve an inflammatory response. Poor clearance has been attributed to defects in neutrophil apoptosis, a process that is regulated in part by reactive oxygen species (ROS) produced by activated neutrophils. Using ROS-deficient and wild-type macrophages treated with extracellular ROS scavengers we demonstrate that macrophage-derived ROS also influence phagocytosis and do so by direct and indirect mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / immunology
  • Cell Separation
  • Flow Cytometry
  • Humans
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Membrane Glycoproteins / deficiency
  • Membrane Glycoproteins / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NADPH Oxidase 2
  • NADPH Oxidases / deficiency
  • NADPH Oxidases / genetics
  • NADPH Oxidases / immunology
  • NADPH Oxidases / metabolism
  • Neutrophils / immunology
  • Neutrophils / pathology
  • Phagocytosis / physiology*
  • Reactive Oxygen Species / immunology*
  • Reactive Oxygen Species / metabolism

Substances

  • Membrane Glycoproteins
  • Reactive Oxygen Species
  • Cybb protein, mouse
  • NADPH Oxidase 2
  • NADPH Oxidases