Indirect immunofluorescence assay for intra vitam diagnosis of avian bornavirus infection in psittacine birds

J Clin Microbiol. 2010 Jun;48(6):2282-4. doi: 10.1128/JCM.00145-10. Epub 2010 Apr 14.

Abstract

Different avian bornavirus (ABV) genotypes have recently been detected in psittacine birds with proventricular dilatation disease (PDD), an inflammatory fatal central and peripheral nervous system disorder. An indirect immunofluorescence assay (IIFA) for intra vitam demonstration of ABV-specific serum antibodies was established since reverse transcription-PCR (RT-PCR) assays may not detect all ABV variants.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Bird Diseases / diagnosis*
  • Bird Diseases / virology
  • Bornaviridae / classification
  • Bornaviridae / isolation & purification*
  • Fluorescent Antibody Technique, Indirect / methods
  • Histocytochemistry
  • Microscopy
  • Mononegavirales Infections / diagnosis
  • Mononegavirales Infections / pathology
  • Mononegavirales Infections / veterinary*
  • Mononegavirales Infections / virology
  • Psittaciformes / virology*
  • Virology / methods*

Substances

  • Antibodies, Viral